Avesani, Simone; Viesi, Eva; Alessandrì, Luca; Motterle, Giovanni; Bonnici, Vincenzo; Beccuti, Marco; Calogero, Raffaele; Giugno, Rosalba
Background: Spatial transcriptomics (ST) combines stained tissue images with spatially resolved high-throughput RNA sequencing. The spatial transcriptomic analysis includes challenging tasks like clustering, where a partition among data points (spots) is defined by means of a similarity measure. Improving clustering results is a key factor as clustering affects subsequent downstream analysis. State-of-the-art approaches group data by taking into account transcriptional similarity and some by exploiting […]
Baccega, Daniele; Pernice, Simone; Terna, Pietro; Castagno, Paolo; Moirano, Giovenale; Richiardi, Lorenzo; Sereno, Matteo; Rabellino, Sergio; Maule, Milena Maria; Beccuti, Marco
Many governments enforced physical distancing measures during the COVID-19 pandemic to avoid the collapse of often fragile and overloaded health care systems. Following the physical distancing measures, school closures seemed unavoidable to keep the transmission of the pathogen under control, given the potentially high-risk of these environments. Nevertheless, closing schools was considered an extreme and the last resort of governments, and so various non-pharmaceutical interventions in schools were […]
Tarallo, Sonia; Ferrero, Giulio; De Filippis, Francesca; Francavilla, Antonio; Pasolli, Edoardo; Panero, Valentina; Cordero, Francesca; Segata, Nicola; Grioni, Sara; Pensa, Ruggero Gaetano; Pardini, Barbara; Ercolini, Danilo; Naccarati, Alessio
Objectives MicroRNA (miRNA) profiles have been evaluated in several biospecimens in relation to common diseases for which diet may have a considerable impact. We aimed at characterising how specific diets are associated with the miRNome in stool of vegans, vegetarians and omnivores and how this is reflected in the gut microbial composition, as this is still poorly explored. Design We performed small RNA and shotgun metagenomic sequencing in faecal samples and dietary recording from 120 healthy volunteers, […]
Current Radiopharmaceuticals
Calandri, Enrico; Giraudo, Maria Teresa; Sirovich, Roberta; Ostan, Antonella; Pultrone, Mirco; Frantellizzi, Viviana; Conversano, Lucia; Bagnasacco, Paolo; Gallina, Sonya; De Vincentis, Giuseppe
Background: An accurate measurement of the target volume is of primary importance in theragnostics of hyperthyroidism. Objective: Our purpose was to evaluate the accuracy of a threshold-based isocontour extraction procedure for thyroid tissue volumetry from SPECT-CT. Methods: Cylindrical vials with a fixed volume of 99mTcO4 at different activities were inserted in-to a neck phantom in two different thickness settings. Images were acquired by orienting the phantom in different positions, i.e., 40 […]
De Cobelli, Francesco; Calandri, Marco; Della Corte, Angelo; Sirovich, Roberta; Gazzera, Carlo; Della Vigna, Paolo; Bonomo, Guido; Varano, Gianluca Maria; Maiettini, Daniele; Mauri, Giovanni; Camisassi, Nicola; Steidler, Stephanie; Ratti, Francesca; Gusmini, Simone; Ronzoni, Monica; Aldrighetti, Luca; Odisio, Bruno C.; Racca, Patrizia; Fonio, Paolo; Veltri, Andrea; Orsi, Franco
Objectives: Oligometastatic colorectal cancer benefits of locoregional treatments but data concerning microwave ablation (MWA) are limited and interactions with systemic therapy are still debated. The aim of this study is to evaluate safety and effectiveness of Thermosphere™ MWA (T-MWA) of colorectal liver metastases (CLM) and factors affecting local tumor progression-free survival (LTPFS). Methods: In this multi-institutional retrospective study (January 2015–September 2019), patients who underwent […]
Communications in Statistics: Simulation and Computation
Giraudo, Maria Teresa; Ricceri, Fulvio; Rosso, Elena
GigaScience
Avesani, Simone; Viesi, Eva; Alessandrì, Luca; Motterle, Giovanni; Bonnici, Vincenzo; Beccuti, Marco; Calogero, Raffaele; Giugno, Rosalba
Background: Spatial transcriptomics (ST) combines stained tissue images with spatially resolved high-throughput RNA sequencing. The spatial transcriptomic analysis includes challenging tasks like clustering, where a partition among data points (spots) is defined by means of a similarity measure. Improving clustering results is a key factor as clustering affects subsequent downstream analysis. State-of-the-art approaches group data by taking into account transcriptional similarity and some by exploiting […]
JASSS
Baccega, Daniele; Pernice, Simone; Terna, Pietro; Castagno, Paolo; Moirano, Giovenale; Richiardi, Lorenzo; Sereno, Matteo; Rabellino, Sergio; Maule, Milena Maria; Beccuti, Marco
Many governments enforced physical distancing measures during the COVID-19 pandemic to avoid the collapse of often fragile and overloaded health care systems. Following the physical distancing measures, school closures seemed unavoidable to keep the transmission of the pathogen under control, given the potentially high-risk of these environments. Nevertheless, closing schools was considered an extreme and the last resort of governments, and so various non-pharmaceutical interventions in schools were […]
Gut
Stool microRNA profiles reflect different dietary and gut microbiome patterns in healthy individuals
Tarallo, Sonia; Ferrero, Giulio; De Filippis, Francesca; Francavilla, Antonio; Pasolli, Edoardo; Panero, Valentina; Cordero, Francesca; Segata, Nicola; Grioni, Sara; Pensa, Ruggero Gaetano; Pardini, Barbara; Ercolini, Danilo; Naccarati, Alessio
Objectives MicroRNA (miRNA) profiles have been evaluated in several biospecimens in relation to common diseases for which diet may have a considerable impact. We aimed at characterising how specific diets are associated with the miRNome in stool of vegans, vegetarians and omnivores and how this is reflected in the gut microbial composition, as this is still poorly explored. Design We performed small RNA and shotgun metagenomic sequencing in faecal samples and dietary recording from 120 healthy volunteers, […]
Current Radiopharmaceuticals
Calandri, Enrico; Giraudo, Maria Teresa; Sirovich, Roberta; Ostan, Antonella; Pultrone, Mirco; Frantellizzi, Viviana; Conversano, Lucia; Bagnasacco, Paolo; Gallina, Sonya; De Vincentis, Giuseppe
Background: An accurate measurement of the target volume is of primary importance in theragnostics of hyperthyroidism. Objective: Our purpose was to evaluate the accuracy of a threshold-based isocontour extraction procedure for thyroid tissue volumetry from SPECT-CT. Methods: Cylindrical vials with a fixed volume of 99mTcO4 at different activities were inserted in-to a neck phantom in two different thickness settings. Images were acquired by orienting the phantom in different positions, i.e., 40 […]
European Radiology
De Cobelli, Francesco; Calandri, Marco; Della Corte, Angelo; Sirovich, Roberta; Gazzera, Carlo; Della Vigna, Paolo; Bonomo, Guido; Varano, Gianluca Maria; Maiettini, Daniele; Mauri, Giovanni; Camisassi, Nicola; Steidler, Stephanie; Ratti, Francesca; Gusmini, Simone; Ronzoni, Monica; Aldrighetti, Luca; Odisio, Bruno C.; Racca, Patrizia; Fonio, Paolo; Veltri, Andrea; Orsi, Franco
Objectives: Oligometastatic colorectal cancer benefits of locoregional treatments but data concerning microwave ablation (MWA) are limited and interactions with systemic therapy are still debated. The aim of this study is to evaluate safety and effectiveness of Thermosphere™ MWA (T-MWA) of colorectal liver metastases (CLM) and factors affecting local tumor progression-free survival (LTPFS). Methods: In this multi-institutional retrospective study (January 2015–September 2019), patients who underwent […]